基质金属蛋白酶2(MMP2)重组蛋白
Recombinant Matrix Metalloproteinase 2 (MMP2)
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
- 编号RPA100Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU per 1μg
- 亚细胞定位细胞膜, 细胞核, 分泌, 细胞外基质
- 预测分子量25.8kDa
- 实际分子量27kDa(差异分析请参阅说明书)
- 片段与标签Tyr445~Cys662 with N-terminal His Tag
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点5.3
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1296 ¥ 3240 ¥ 6480 ¥ 19440 ¥ 48600
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
![](http://static.cloud-clone.cn/product/pictures/RPA100Mu01-202310301549576545.png)
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA100Mu01 | 基质金属蛋白酶2(MMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA100Mu01 | 基质金属蛋白酶2(MMP2)活性蛋白 | Cell culture; Activity Assays. |
RPA100Mu02 | 基质金属蛋白酶2(MMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA100Mu01 | 基质金属蛋白酶2(MMP2)多克隆抗体 | WB; IHC; ICC; IP. |
PAA100Mu02 | 基质金属蛋白酶2(MMP2)多克隆抗体 | WB; IHC; ICC; IP. |
SEA100Mu | 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA100Mu | 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA100Mu | 基质金属蛋白酶2(MMP2)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA100Mu | 基质金属蛋白酶2(MMP2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Moleculer Vision | Bone morphogenetic protein-2: a potential regulator in scleral remodeling [PubMed: PMC2605409] |
Neuroimmunomodulation | Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source] |
Zahedan Journal of Research in Medical Sciences | The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men [Zjrms: Source] |
Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source] |
Experimental & Translational Stroke Medicine | Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke [PubMed: 21679435] |
PLoS One | Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 [PubMed: PMC3797697] |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
Clinical and Investigative Medicine | The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells [Pubmed: 23544611] |
Physiol. Res. | The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818] |
Acta Histochemica | RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells [Pubmed: 24681113] |
Peritoneal Dialysis | Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients? [Pubmed: 23994841] |
Polski Przeglad Chirurgiczny | Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course [Pubmed: 23585206] |
Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
Int Immunopharmacol.? | Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis [Pubmed:25824718] |
Journal of Perinatology | Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion [Pubmed:25078863] |
Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
Stem Cells International | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro [Pubmed:25767513] |
Physiology and Pharmacology | A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men [Phypha:Source] |
Mediators of Inflammation | Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse [Journals: Mi] |
Journal of Biomaterials and Tissue Engineering | MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 [Content: Asp] |
Biomedical Materials | In vivo [PubMed: 25634132] |
Int J Clin Exp Pathol | Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways [PubMed: 26191138] |
Hattori and Ishihara Stem Cell Research & Therapy | Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells [PubMed: 25884474] |
Biosens Bioelectron | Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+ [PubMed: 26433066] |
Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140] |
Canadian Journal of Physiology and Pharmacology | Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats [Doi: Abs] |
PLASTIC AND AESTHETIC RESEARCH | A comparative study to evaluate the effect of limited access dressing on diabetic ulcers [Article: 2347-9264] |
Int J Mol Sci | Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ [PubMed: 26703570] |
Basic Research | CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma [Journal:27699] |
International Journal of Oncology | 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway [Pubmed:26782341] |
International Journal of Pharmacology | Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration [profile:57162df608aebf0697f1843d] |
Journal of Stroke and Cerebrovascular Diseases | Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats [Pubmed:26851971] |
VirusDisease | Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446] |
Cell Physiol Biochem. | MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 [Pubmed:27160777] |
Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
Biosensors and Bioelectronics | Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates [science:S0956566315304474] |
world journal of surgery | Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia. [pubmed:28050662] |
disease markers | Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. [pubmed:28348451] |
Balkan Medical Journal | Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage [pubmed:27761275] |
Microvascular Research | Pleiotropic effects of survivin in vascular endothelial cells. [pubmed:27373518] |
BMC Veterinary Research | Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. [pubmed:27938355] |
Cellular Physiology and Biochemistry | MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. [pubmed:27160777] |
Journal of Biomaterials and Tissue Engineering | MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line [publication:306067745] |
World Journal of Plastic Surgery | Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings. [pubmed:27853690] |
Journal of Biomaterials and Tissue Engineering | Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts [art00008] |
Human cell | Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity [pubmed:28091828] |
Human Cell | MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 [pubmed:27743351] |
Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422] |
Human Cell | MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 [pubmed:28573504] |
Cellular Physiology and Biochemistry | The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019] |
International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748] |
Fundamental & Clinical Pharmacology | Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 [DOI:10.1111/fcp.12294] |
PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis [pubmed:28665955] |
Nanotoxicology | Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway [10.1080/17435390.2017.1357213] |
Archives of Medical Science | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. [pubmed:28507585] |
Molecular Medicine Reports | Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis [pubmed:28901438] |
European Journal of Medicinal Chemistry | Syntenin-targeted peptide blocker inhibits progression of cancer cells [Pubmed:29857221] |
Journal of Neurochemistry | Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling [Pubmed:29572844] |
Journal of Cellular Biochemistry | Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia … [10.1002:jcb.26849] |
Inflammation | Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy [Pubmed:29704151] |
OncoTargets and Therapy | sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion [Pubmed:30022839] |
Biomed Research International | CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling [Pubmed:29984229] |
APMIS | Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide [Pubmed:30058214] |
Molecular Medicine Reports | Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitro [Pubmed:29328476] |
Oncology Letters | miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells [10.3892:ol.2018.9170] |
Taiwanese Journal of Obstetrics & Gynecology | HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663] |
Open Medicine | MMP-2 and TIMP-2 in patients with heart failure and chronic kidney disease [Pubmed:29915813] |
Pediatric Research | Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. [Pubmed: 30464333] |
Advanced Healthcare Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
Cellular & Molecular Biology Letters | MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1 [] |
Mediators of Inflammation | Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma [] |
Journal of endovascular therapy: an official journal of the International Society of Endovascular Specialists | Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular … [Pubmed: 31464166] |
Oncology Research | MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 [Pubmed: 31796150] |
Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
Acta Biomaterialia | Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial Cells [Pubmed: 32205213] |
JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
JOURNAL OF ETHNOPHARMACOLOGY | Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling … [Pubmed: 32745509] |
heliyon | Matrix levels of metalloproteinase-2 (MMP-2) and toxicity evaluation of carbonate apatite-based endodontic sealer in rat subcutaneous implantation [Pubmed: 32671254] |
J Cell Physiol | A novel role of VEPH1 in regulating AoSMC phenotypic switching [Pubmed: 32342520] |
Advances in Animal and Veterinary Sciences | Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats [] |
Alteration on MMP-2 Levels in Women Diabetes Patients in Basrah [] | |
Veterinary Sciences: Research and Reviews | Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep [] |
MEDIATORS OF INFLAMMATION | MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic [33488296] |
Oncol Lett | GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression [33613720] |
Journal of Pregnancy | Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia [33381317] |
Immunopharmacol Immunotoxicol | Tofacitinib enhances remyelination and improves myelin integrity in cuprizone-induced mice [34618622] |
Exp Cell Res | Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human … [34762897] |
Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer [34650982] | |
Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980] |
Journal of Pharmaceutical Research International | Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid … [] |
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … [] | |
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes [] |
留言咨询