半乳糖凝集素1(GAL1)多克隆抗体
Polyclonal Antibody to Galectin 1 (GAL1)
LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
- 编号PAA321Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA321Hu01-半乳糖凝集素1(GAL1)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Mus musculus (Mouse，小鼠)
- 应用WB,IHC; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Porcine Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GAL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Rectum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Renal cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Colon Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Uterus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GAL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GAL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: K562 cell
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant GAL1, Human.
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Knockout Varification:
Lane 1: Wild-type K562 cell lysate;
Lane 2: GAL1 knockout K562 cell lysate;
Predicted MW: 15kd
Observed MW: 15kd
Primary Ab: 1µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: K562 cell lysate; Lane2: Mouse Heart lysate; Lane3: Mouse Liver lysate; Lane4: Mouse Kidney lysate
Primary Ab: 1µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MDA-MB-231 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human GAL1 Polyclonal Antibody (Catalog PAA321Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GAL1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GAL1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA321Hu01 | 半乳糖凝集素1(GAL1)活性蛋白 | Cell culture; Activity Assays. |
| RPA321Hu01 | 半乳糖凝集素1(GAL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA321Hu01 | 半乳糖凝集素1(GAL1)多克隆抗体 | WB,IHC; FCM |
| FAA321Hu01 | 抗半乳糖凝集素1(GAL1)多克隆抗体 | Flow cytometry. |
| LAA321Hu71 | 半乳糖凝集素1(GAL1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA321Hu81 | 半乳糖凝集素1(GAL1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA321Hu22 | 半乳糖凝集素1(GAL1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA321Hu21 | 半乳糖凝集素1(GAL1)单克隆抗体 | WB |
| MEA321Hu | 半乳糖凝集素1(GAL1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA321Hu | 半乳糖凝集素1(GAL1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA321Hu | 半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA321Hu | 半乳糖凝集素1(GAL1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA321Hu01 | 半乳糖凝集素1(GAL1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| International Journal of Oncology | Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans [Pubmed:24589677] |
| Clinica Chimica Acta | Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [Pubmed:25578395] |
| Journal of Perinatal Medicine | Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth [PubMed: 24717333] |
| Biomaterials | Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy [Pubmed:29272764] |
| Advances in Experimental Medicine and Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
| RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis [Pubmed: 31678459] |
| Eur J Nucl Med Mol Imaging | Galectin expression detected by 68Ga-galectracer PET as a predictive biomarker of radiotherapy resistance [Pubmed:35106644] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771] |
| In Vitro Bovine Embryo Production Using Medium's Supplemented with Recombinant Human Galectin-1 Buffered [] |
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