视黄醇结合蛋白4(RBP4)活性蛋白
Active Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- 编号APA929Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点6.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
活性实验
![](http://static.cloud-clone.cn/product/pictures/APA929Mu01-202406031605406876.jpg)
Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant mouse RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 µL of 50 µg/mL rmRBP4 and 12.5 µL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 µL of 50 µg/mL rmRBP4 and 12.5 µL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is > 4.6 µM.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA929Mu01 | 视黄醇结合蛋白4(RBP4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA929Mu01 | 视黄醇结合蛋白4(RBP4)活性蛋白 | Cell culture; Activity Assays. |
RPA929Mu02 | 视黄醇结合蛋白4(RBP4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA929Mu02 | 视黄醇结合蛋白4(RBP4)多克隆抗体 | WB; IHC; ICC; IP. |
PAA929Mu01 | 视黄醇结合蛋白4(RBP4)多克隆抗体 | WB; IHC; ICC; IP. |
LAA929Mu81 | 视黄醇结合蛋白4(RBP4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA929Mu71 | 视黄醇结合蛋白4(RBP4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA929Mu | 视黄醇结合蛋白4(RBP4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA929Mu | 视黄醇结合蛋白4(RBP4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA929Mu01 | 视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078] |
Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593] |
Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504] |
Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381] |
J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061] |
Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608] |
Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126] |
Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311] |
Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. [pubmed:27446311] |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009] |
Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416] |
Molecular Medicine Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949] |
Relationship between retinol and risk of diabetic retinopathy: a case-control study [] | |
Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589] |
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals [] | |
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma [] | |
Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861] |
Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274] |
Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507] |