脂质运载蛋白2(NGAL)活性蛋白
Active Neutrophil gelatinase-associated lipocalin (NGAL)
Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
- 编号APB388Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点9.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1272 ¥ 3180 ¥ 6360 ¥ 19080 ¥ 47700
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活性实验
![](http://static.cloud-clone.cn/product/pictures/APB388Hu01-202402021557179258.jpg)
Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). The activity assay of recombinant human NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant human NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 µL of the different concentrations of Fe(DHBA)3 and 50 µL of 100 ug/ml rmNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant human Lipocalin-2 can bind >8 µM of Fe(DHBA)3.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB388Hu02 | 脂质运载蛋白2(NGAL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB388Hu61 | 脂质运载蛋白2(NGAL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB388Hu01 | 脂质运载蛋白2(NGAL)活性蛋白 | Cell culture; Activity Assays. |
RPB388Hu01 | 脂质运载蛋白2(NGAL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB388Hu01 | 脂质运载蛋白2(NGAL)多克隆抗体 | WB; IHC; ICC; IP. |
PAB388Hu02 | 脂质运载蛋白2(NGAL)多克隆抗体 | WB; IHC; ICC; IP. |
LAB388Hu81 | 脂质运载蛋白2(NGAL)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAB388Hu71 | 脂质运载蛋白2(NGAL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB388Hu23 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
MAB388Hu22 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
MAB388Hu24 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
MAB388Hu21 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB |
MAB388Hu25 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
SEB388Hu | 脂质运载蛋白2(NGAL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB388Hu | 脂质运载蛋白2(NGAL)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB388Hu | 脂质运载蛋白2(NGAL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSB388Hu01 | 脂质运载蛋白2(NGAL)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB388Hu01 | 脂质运载蛋白2(NGAL)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury [33604384] |
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