结缔组织生长因子(CTGF)活性蛋白
Active Connective Tissue Growth Factor (CTGF)
CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2
- 编号APA010Hu03
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 80%
- 等电点7.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. As CTGF has the function of cell adhesion, we measure the activity of recombinant human CTGF by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cell. When 5 x 104 cells/well are added to different concentrations of recombinat human CTGF coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of Balb/3T3 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.6 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA010Hu04 | 结缔组织生长因子(CTGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA010Hu02 | 结缔组织生长因子(CTGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA010Hu01 | 结缔组织生长因子(CTGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA010Hu03 | 结缔组织生长因子(CTGF)活性蛋白 | Cell culture; Activity Assays. |
| RPA010Hu03 | 结缔组织生长因子(CTGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA010Hu21 | 结缔组织生长因子(CTGF)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA010Hu08 | 结缔组织生长因子(CTGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA010Hu02 | 结缔组织生长因子(CTGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA010Hu01 | 结缔组织生长因子(CTGF)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA010Hu71 | 结缔组织生长因子(CTGF)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA010Hu81 | 结缔组织生长因子(CTGF)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA010Hu24 | 结缔组织生长因子(CTGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA010Hu22 | 结缔组织生长因子(CTGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA010Hu23 | 结缔组织生长因子(CTGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA010Hu21 | 结缔组织生长因子(CTGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA010Hu28 | 结缔组织生长因子(CTGF)单克隆抗体 | WB; IHC; ICC; IP. |
| LAA010Hu82 | 结缔组织生长因子(CTGF)单克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA010Hu72 | 结缔组织生长因子(CTGF)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA010Hu | 结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA010Hu | 结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA010Hu | 结缔组织生长因子(CTGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA010Hu01 | 结缔组织生长因子(CTGF)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA010Hu01 | 结缔组织生长因子(CTGF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Orthopaedic Research | Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis model [PubMed: 19824055] |
| World Journal of Gastroenterology | Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function [PubMed: PMC2726461] |
| PLos One | Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound Healing [Plosone: 0032128] |
| Clinical Biochemistry | Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy [ScienceDirect: S0009912012000987] |
| The Journal of Heart and Lung Transplantation | Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development? [PubMed: S1053249812010121] |
| Biomedical and Environmental Sciences | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats [Besjournal: Source] |
| Disease Markers | Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosis [PubMed: 22846210] |
| World Journal of Gastroenterology | Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis [PubMed: PMC3351780] |
| Journal of ethnopharmacology | Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397] |
| Radiation Oncology | Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy [Ro-journal: Source] |
| PLoS One. | Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice Model [Pubmed:Pmc4157792] |
| European Journal of Heart Failure | Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal models [Pubmed:24464932] |
| Bone | Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic Lineage [Pubmed:24792956] |
| American Journal of Pathology | Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis [Pubmed:25701883] |
| J Gastroenterol | Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis [PubMed: 26190501] |
| Life Sciences | Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitus [Pubmed:27049870] |
| International Journal of Molecular Sciences | TGF-β and Physiological Root Resorption of Deciduous Teeth. [pubmed:28035998] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| British Journal of Ophthalmology | The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence [pubmed:27531356] |
| Pathophysiology | Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases [pubmed:27686729] |
| Evidence-Based Complementary and Alternative Medicine | Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway [pubmed:28698735] |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| Life Sciences | Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activation [Pubmed:29107793] |
| International Journal of Psychophysiology | Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller Cells [Pubmed:30123889] |
| Journal of Cellular Physiology | TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretion [Pubmed: 30426494] |
| Journal of Clinical Medicine | Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with … [Pubmed: 30413105] |
| Nature Communications | Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M [Pubmed: 31249305] |
| Cardiology | Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059] |
| Stem Cells International | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging [Pubmed: 31827530] |
| Pharmaceuticals | Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced Nephrotoxicity [Pubmed: 33212804] |
| BMC Veterinary Research | Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the … [Pubmed: 32943074] |
| Revista Portuguesa de Cardiologia | MicroRNAs and ventricular remodeling in aortic stenosis [Pubmed: 32682570] |
| Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction [] | |
| Exp Gerontol | High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth … [34455071] |
| Research Square | Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway [] |
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